5L Laboratory scale bioreactor

Novel magnetic coupling drive to improve sterility and retain sterility throughout the fermentation process

Reliable and adaptable Siemens Simatic S7-1500 based PLC to control the bioprocesses and maintain life in the reactor

Fed-batch according adjusted profiles

Fed-batch according adjusted profiles

No welded lid components – ideal for cleaning and sterility

To specify reactor design and price please contact us:

EDF-5.4 is an autoclavable glass bioreactor with working volume till 4,5l (total volume 6,2 l, inside diameter 150 mm).

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Laboratory bioreactor EDF-5.4 has two versions:

• EDF5.4_1 for the cultivation of bacteria, yeasts and mycelial cultures;

• EDF-5.4_2 for the cultivation of mammalian and plant cells.

EDF-5.4 has a compact, ergonomic and robust design. Glass cylinder vessel is mounted between the upper lid and the metallic jacketed bottom. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular,
washing and autoclaving.

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Extension and option possibilities

Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.

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Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.

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On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).

Collapsible condenser allows decrease the height during autoclaving

Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions

Model

EDF-5.4_1 for Microbiology

EDF-5.4_2 for cell cultures

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Vessel

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Total volume (L) 6.2

Working volume (L) 2 – 4,5

Inside diameter/Inside height (mm) 150/350

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Aeration, mixing

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Controls - Rotameter, TMFC (optional)

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Aeration

Air, + O2 

For EDF-5.4_2 Air / CO2, + O2, N2 (optional) Air / CO2 in excess space

Range, l / min

EDF-5.4_1   0.5 – 10

EDF-5.4_2   0.1 – 2 (other options available)

Bubbler

EDF-5.4_1 Ring Bubbler

EDF-5.4_2 Microsparger

Filters

D51 mm 0.2 μm PTFE

Off-gas condenser

Water cooling

Drive unit type

Top placed magnetic drive

Mixer rotation speed (rpm)

40 - 1300

Impellers

EDF-5.4_1   Two standard Rushton turbines

EDF-5.4_2  Stirrers with inclined "propeller" blades

Control

Siemens Simatic S7-1500

Control Panel

Touch Screen Weintek eM3070, 5,7” TFT

Temperature

Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C

pH

Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units

pO2

EDF-5.4_1 Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1%

EDF-5.4_2 Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).

Foam

Conductivity sensor

Feeding

0.05-50 ml/min according to the set profile

Peristaltic pumps

4 built-in variable speed pumps. Additional pumps (optional)

Dimensions

Overall dimensions of the bioreactor (mm) 930(W) х 800(H) х 600(D)

Fully equipped and ready for work