
5L Laboratory scale bioreactor


Novel magnetic coupling drive to improve sterility and retain sterility throughout the fermentation process

Reliable and adaptable Siemens Simatic S7-1500 based PLC to control the bioprocesses and maintain life in the reactor


Fed-batch according adjusted profiles
Fed-batch according adjusted profiles

No welded lid components – ideal for cleaning and sterility
To specify reactor design and price please contact us:
EDF-5.4 is an autoclavable glass bioreactor with working volume till 4,5l (total volume 6,2 l, inside diameter 150 mm).
Laboratory bioreactor EDF-5.4 has two versions:
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EDF5.4_1 for the cultivation of bacteria, yeasts and mycelial cultures;
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EDF-5.4_2 for the cultivation of mammalian and plant cells.
EDF-5.4 has a compact, ergonomic and robust design. Glass cylinder vessel is mounted between the upper lid and the metallic jacketed bottom. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular,
washing and autoclaving.
Extension and option possibilities
Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.
Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.
On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).

Collapsible condenser allows decrease the height during autoclaving

Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions
Model
EDF-5.4_1 for Microbiology
EDF-5.4_2 for cell cultures
Vessel
Total volume (L) 6.2
Working volume (L) 2 – 4,5
Inside diameter/Inside height (mm) 150/350
Aeration, mixing
Controls - Rotameter, TMFC (optional)
Aeration
Air, + O2
For EDF-5.4_2 Air / CO2, + O2, N2 (optional) Air / CO2 in excess space
Range, l / min
EDF-5.4_1 0.5 – 10
EDF-5.4_2 0.1 – 2 (other options available)
Bubbler
EDF-5.4_1 Ring Bubbler
EDF-5.4_2 Microsparger
Filters
D51 mm 0.2 μm PTFE
Off-gas condenser
Water cooling
Drive unit type
Top placed magnetic drive
Mixer rotation speed (rpm)
40 - 1300
Impellers
EDF-5.4_1 Two standard Rushton turbines
EDF-5.4_2 Stirrers with inclined "propeller" blades
Control
Siemens Simatic S7-1500
Control Panel
Touch Screen Weintek eM3070, 5,7” TFT
Temperature
Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C
pH
Hamilton or Mettler Toledo sensor. Acid/Alkali 0-14 +/- 0.01 pH units
pO2
EDF-5.4_1 Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1%
EDF-5.4_2 Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).
Foam
Conductivity sensor
Feeding
0.05-50 ml/min according to the set profile
Peristaltic pumps
4 built-in variable speed pumps. Additional pumps (optional)
Dimensions
Overall dimensions of the bioreactor (mm) 930(W) х 800(H) х 600(D)

Fully equipped and ready for work