This bioreactor line is for the cultivation of Pichia pastoris to manufacture enzymes and proteins.
The system is fully closed and automated, consisting of following key elements:

1. 50, 500 and 2×5000 litre bioreactors (total volumes);

2. Stationary CIP/SIP for automated cleaning and sterilization of bioreactors, reactors and
   connecting pipelines;

3. Circulation sterilizer for sterilization of substrate;

4. Feeding reactors for glycerol and methanol;

5. Connecting heat-insulated pipelines and the group of pneumatically controlled membrane valves.

The following parameters in bioreactors are controlled:

• temperature;

• pH;

• DO;

• overpressure;

• hydrostatic pressure;

• level;

• methanol concentration;

• foaming.

The central cascade control system includes SCADA
developed in compliance with the requirements of 21 CFR Part 11.