5L Laboratory scale photobioreactor
Photobioreactor PBR-5.4 is an autoclavable glass / stainless steel photobioreactor with vessel volume of 6.2 L (working volume 2 – 4.5 L, inner diameter – 150 mm).
Laboratory bioreactor PBR-5.4 is devised for precise phototrophic cultivation of algae and cyanobacteria.
Bioprocess controller BIO-4
In the bioprocess controller BIO-4 are included: colored 5.6” touch screen interface display, thermostat, peristaltic pumps, LED cover, illumination control, air and СO2 gas supply unit.
Bioprocess controller BIO-4 ensures:
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Control of pH, pO2, illumination (LED) intensity, temperature, foam and culture level
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Display of the process data (temperature, pH, pO2, illumination intensity etc.)
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CO2 supply via automatic pH control or manual CO2 gas flow rate control
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Illumination intensity control – 0 to 100% (0 to 1500 µmol/m2/s)
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Control of the illumination intensity as a time dependent profile program
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Illumination day/night cycles
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Remote control of the experiment via internet, real time data acquisition and plotting (SCADA Control System)
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Thermoregulation (is carried out trough double wall bottom (jacket), with thermo-stating water circulation)
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Both static and day/night temperature control is possible
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Mixing (provided by mixer consisting of 2 propeller type turbines devised for shear sensitive microorganism cultivation
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Addition of various substances (provided by peristaltic pumps for hemostat cultivation mode and fed-batch control (fed-batch control constant or pulse).
Extension and option possibilities
Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.
Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.
On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).
Novel magnetic coupling drive to improve sterility and retain sterility throughout the fermentation process
Reliable and adaptable Siemens Simatic S7-1500 based PLC to control the bioprocesses and maintain life in the reactor
Fed-batch according adjusted profiles
Fed-batch according adjusted profiles
Collapsible condenser allows decrease the height during autoclaving
Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions
Model
PBR-5.4
Vessel
Total volume (L) 6.2
Working volume (L) 2 – 4,5
Inside diameter/Inside height (mm) 150/350
Aeration, mixing
Controls - Rotameter, TMFC (optional)
Aeration
Air, + O2 (optional) or Air / CO2, + O2, N2 (optional) Air / CO2 in excess space
Range, l / min
0.5 – 10
Bubbler
Ring Bubbler
Filters
D51 mm 0.2 μm PTFE
Off-gas condenser
Water cooling
Drive unit type
Top placed magnetic drive
Mixer rotation speed (rpm)
40 - 1000
Impellers
Two standard Rushton turbines or Stirrers with inclined "propeller" blades
Control
Siemens Simatic S7-1500
Control Panel
Touch Screen Weintek eM3070, 5,7” TFT
Temperature
Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C
pH
Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units
pO2
Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1% or Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).
Foam
Conductivity sensor
Feeding
0.05-50 ml/min according to the set profile
Peristaltic pumps
4 built-in variable speed pumps. Additional pumps (optional)
Dimensions
Overall dimensions of the bioreactor (mm) 930(W) х 800(H) х 600(D)
No welded lid components – ideal for cleaning and sterility