15L Laboratory scale bioreactor

15L bioreactor
magnetic mixer bioreactor 15L

Novel magnetic coupling drive to improve sterility and retain sterility throughout the fermentation process

bioreactor siemens simatic

Reliable and adaptable Siemens Simatic S7-1500 based PLC to control the bioprocesses and maintain life in the reactor

15L bioreactor interface fed batch automation
15L bioreactor SCADA

Fed-batch according adjusted profiles

Fed-batch according adjusted profiles

15L bioreactor top lid

No welded lid components – ideal for cleaning and sterility

To specify reactor design and price please contact us:

EDF-15.1 is autoclavable glass bioreactor with a working volume of 11 liters (total volume of 16 liters, the internal diameter of 200 mm).

Bioreactor is foreseen for culturing bacteria, yeast, and mycelia microorganism cultures.

However bioreactor EDF-15.1 can be adapted for culturing mammalian, stem and plant cells.  Depending on the applied microbial culture it is possible to adapt the configuration of the bioreactor in a corresponding way by replacing stirrer and a bubbling system complementing aeration gas mixing unit (air, O2, N2, CO2).

EDF-15.1 has a compact and ergonomic design. Glass cylinder alias vessel is mounted between the upper lid and the metal bottom. The bottom of the vessel has a jacket for temperature control of the vessel. It also has an autoclavable sampler for aseptic sampling. The material of the vessel, which is in contact with the product, is Pyrex borosilicate glass and stainless steel 316L. Bioreactor vessel mounted on a stainless steel legs. The lid has a convenient carrying handle. The design of the bioreactor is easy to maintain and apply basic operations and preparatory process, in particular, washing and autoclaving.

Extension and option possibilities


Gas mixing using mass flow controllers Hamilton ARC pH and DO sensors. These sensors send 4-20 mA signals directly to PLC and ensure at the same time information management via Bluetooth in smartphones or PC installed programs. This program generates a report about all calibration procedures, sterilization numbers, predicted service life.

Connection of additional sensors: culture turbidity (optical density), culture permittivity (viable cell density), conductivity, methanol/ethanol, off-gas analysis (O2, CO2, CH4), etc.

On-line/off-line data analysis and advanced process control possibilities using PC implemented program (Matlab, Python) algorithms communicating to process control system (PLC and SCADA).

15L bioreactor condenser

Collapsible condenser allows decrease the height during autoclaving

15L bioreactor double bottom

Jacketed metallic bottom and bottom placed sterilizable sampler creates easy and robust operational conditions

Model

 

EDF-15.1​

Vessel

Total volume (L) 16

Working volume (L) 5-11

Inside diameter/Inside height (mm) 200/500

Aeration, mixing

Controls - Rotameter, TMFC (optional)

Aeration

Supply and mixing two gases (air, O2 or air, CO2) to ensure oxygen or CO2 enrichment in headspace, including 2 pressure reducer with pressure gauges; 2 rotameters (for air 0 – 20 l/min for O2 0 – 10 l/min); microbiological filter; ring sparger (optionally microsparger); inlet gas filter; outlet gas filter with easy bending condenser; membrane valve and filter

OPTIONAL 

Separate gas supply or gas mixture to the surface of the culture

MFC for air, O2 or CO2

Gas mixing (air, N2, O2, CO2)

Bubbler

Ring Bubbler or Microsparger

Filters

D51 mm 0.2 μm PTFE

Off-gas condenser

Water cooling

Drive unit type

Top placed magnetic drive

Mixer rotation speed (rpm)

40 – 700

Impellers

Two standard Rushton turbines or Stirrers with inclined "propeller" blades

Control

Siemens Simatic S7-1500

Control Panel

Touch Screen Weintek eM3070, 5,7” TFT

Temperature

Control limits: from 5°C above cooling water up to 70 °C Measurement accuracy +/- 0.1 °C and control accuracy +/- 0.2 °C

Built-in thermostat (heater – 500 W, chamber, recirculation pump, cooling water valve). Temperature control range from 5 °C till 55 °C above the chiller temperature. Measurement accuracy ± 0,1 °C; accuracy of temperature within the prescribed limits ± 0,2 °C

Ports

For mixer/agitator drive; for pH, pO2, T, foam and level sensors; port with three needles for introducing additives to control the pH and foam level; hemostat tube to collect the product; aseptic pierceable membrane port, a port for the incoming gas with a filter of 0.2 micron, the condenser exhaust gas with a filter of 0.2 um, 2 spare ports closed with plugs

pH

Hamilton or Mettler Toledo sensor. Acid/Alkali 2-12 +/- 0.01 pH units

pO2

 Hamilton or Mettler Toledo sensor. Stir control + О2 (optional). 0-150% +/- 1% or Hamilton or Mettler Toledo sensor. Stir control + gas mixtures (optional).

Foam

Conductivity sensor

Feeding

0.05-50 ml/min according to the set profile

Peristaltic pumps

4 freely configurable built-in pump with digital control (ON / OFF), and an analog supply pump with a constant flow rate of the solution (option)

Dimensions

Overall dimensions of the bioreactor (mm) 1000 (W) х 970 (H) х 600 (D)

The minimum size of the autoclave chamber for vessel autoclaving (mm)

760 (Height) х 320 (Diameter)